This web page was produced as an assignment for Genetics 677, an undergraduate course at UW-Madison.
Protein Domains
Pfam was used to generate an image of the Fgfr2 protein based on its domains for humans and six homologous proteins. This was done by entering the the FASTA sequence of the protein into the sequence search engine. Below are the descriptions of each domain in the human protein as listed by Pfam as well.
Pfam protein image
The human Fgfr2 protein consists of four studied domains. An Ig_2 domain sits closest to the the N-terminus while a Pkinase_Tyr domain sits closest to the C-terminus. In between the protein contains 2 I-set domains.
Ig_2: This indicates an immunoglobulin domain. Immunoglobulin domains are widespread and found in many human proteins with a variety of functions. Although it is not certain what this immunoglobulin domain does in the Fgfr2 protein, Ig domains are suspected to be involved in protein-protein and/or protein-ligand interactions. These domains are made of beta-strands arranged into beta-sheets.
I-set: These domains are also immunoglobulin domains. They are found in several different protein families, but they are known best for their involvement in cell adhesion molecules.
Pkinase_Tyr: Tyrosine kinases are responsible for transferring phosphate groups, and, therefore, are key to many cellular functions.
Ig_2: This indicates an immunoglobulin domain. Immunoglobulin domains are widespread and found in many human proteins with a variety of functions. Although it is not certain what this immunoglobulin domain does in the Fgfr2 protein, Ig domains are suspected to be involved in protein-protein and/or protein-ligand interactions. These domains are made of beta-strands arranged into beta-sheets.
I-set: These domains are also immunoglobulin domains. They are found in several different protein families, but they are known best for their involvement in cell adhesion molecules.
Pkinase_Tyr: Tyrosine kinases are responsible for transferring phosphate groups, and, therefore, are key to many cellular functions.
Homologous protein images
Analysis
The human protein domains are very similar to those of the homologs. Each homolog has at least 2 I-set domains and a Tyrosine Kinase domain, just like the human. In addition, an Ig_2 (like in the human) or an additional I-set domain, is present in all but the dog homolog. Since Ig_2 and I-set domains are both immunoglobulin domains, they are probably capable of similar tasks so having one or the other in the first domain position likely has little effect. It is unclear why the dog protein does not have another I-set or an Ig_2 domain.